help with ASO PROBES - genetics

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Posted by LIS from IP 24.174.230.188 on September 01, 2010 at 12:38:51:

someone help me pls,,..
I'm studying genetics and i don't understand this question about ASO probe...

the most commom mutation for cystic fibrosis is deletion F508, which can e detected using ASO. if the normal and mutant sequences for this region are:

NORMAL 5' AAG ATT TTT TGT ACC 3'
MUTANT 5' AAG ATT TGT ACC 3'

Which of the following probes would most likely be used to detect the disease?

answer: 5' GGT ACA AAT CTT 3'

pls somebody answer!!! :D

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