PCR >> Southern blot

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Posted by Sidd from IP 66.171.246.115 on February 28, 2006 at 23:21:08:

In Reply to: nbme-I question -- need help posted by xy on February 28, 2006 at 17:25:58:

:) I think i was wrong and Hossein was right. I realised this after reading his answer. The question makes no mention of enzymes or free nucleotides in the medium.

Its probably a southern blot, but i think the answer is still d. If considerable base mismatch occurs (a), wouldnt you end up with a really low specificity test? I thought the blots were highly specific (e.g. Western blot for HIV).

Wonder what you think Hossein.

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