Posted by Sidd from IP 66.171.246.115 on February 28, 2006 at 23:07:55:
In Reply to: nbme-I question -- need help posted by xy on February 28, 2006 at 17:25:58:
This is the classic PCR. You heat the DNA to an EXACT temperature (121C? something in that range) seperate the strands, along with a heat resistant DNA polymerase derived from Thermophilic bacteria. Cool it and you form a complimentary DNA strand on each of the original strands. Repeat it 30 or so times, lots of DNA to do whatever you want: make vectors, study protein expression, can also be used to make probes if you have the right primers.
The whole point being, you want EXACT copies of the original DNA. I think by "high stringency conditions", they mean conditions that as present during PCR (assumption), so im wiling to go with d.